The contamination problem of Bacillus cereus seriously affects the food safety of China, the development of prevention and control technology against Bacillus cereus is urgent. Lactic acid bacteria, as a safe and efficient biological control method, have a good prospect in controlling Bacillus cereus precisely. Previous studies have found that Lactiplantibacillus, Lactobacillus and Lacticaseibacillus are the main genus of antagonistic bacteria. The antibacterial components against Bacillus cereus are mainly bacteriocins, organic acids and extracellular polysaccharides. The antagonistic mechanism mainly included destroying the integrity of cell membrane, inhibiting spore germination, biofilm formation and toxin expression, but the mechanism of internal gene expression regulation is still lack of in-depth exploration. This paper reviewed the progress in controlling of Bacillus cereus by lactic acid bacteria, summarized the lactic acid bacteria species, antibacterial compounds and their antagonistic mechanisms against Bacillus cereus, as well as the applications in food, also prospected the investigation of new types of microbial antibacterial components, the mechanism of action and the future applications, to provide new insights for the development of green prevention and control technologies of foodborne pathogens.

Foodborne pathogenic bacteria are one of the main causes of foodborne illness, and pose a serious threat to global public health. At present, the detection of pathogenic bacteria in food primarily relies on conventional plate culture method and auxiliary means, such as chromogenic medium and biochemical identification. However, these methods are long-term, cumbersome and prone to false positives, making them inadequate to meet the demands of modern food safety for rapid and accurate detection. Therefore, the development of rapid and effective detection technologies is crucial for the timely detection, control and elimination of bacterial contamination. This paper took several common foodborne pathogenic bacteria, including Escherichia coli, Salmonella Typhimurium, Listeria monocytogenes, and Staphylococcus aureus, as examples to provide a detailed analysis of the principles, applications, advantages and limitations of various detection methods, and discussed the development trend of detection technology in the future. This study provides both theoretical support and practical guidance for enhancing existing detection methods and the developing new rapid detection technologies, with the goal of more effectively tackling challenges related to food safety.

Objective To detect and analyze the residue conditions of 57 kinds of pesticide in commercially available fruits in Handan Area from 2020 to 2024 and assess the health risks of pesticide residues for local residents. Methods Followed QuEChERS extraction, samples were analyzed using liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry. Chronic dietary exposure was assessed via a Monte Carlo simulation-based risk model, whereas acute risk was determined by the acute reference dose. Results The p detection rate of pesticide types in 80 fruit samples was 28.1% with an overall item detection rate of 1.12% and overall sample detection rate of 33.8% in 80 fruit samples. Dimethoate residues exceeded national standards in 6 cherry and 3 jujube samples. Two or more pesticides were detected in 16.2% of the samples. No highly or extremely toxic pesticides were detected. The chronic dietary intake risk of dimethoate was 1.81, exceeded the safe range, while the chronic dietary intake risk of other pesticides were within acceptable limits. The hazard index value was 1.87, exceeded the safe range. Acute dietary risks for all detected pesticides were within safe ranges. Dimethoate and endosulfan were identified as higher-risk pesticides in both acute and chronic assessments. Conclusion The dietary intake risk of pesticide residues in Handan City (2020—2024) is generally within safe limits. However, there are cases of dimethoate exceeding the standard and multiple pesticides detected in the same sample. Dimethoate posed chronic dietary intake risk and the relevant authorities need to maintain ongoing attention.

With the growing awareness of public health, cereal and coarse grain foods, owing to their superior nutritional value and health-promoting functions, have increasingly become a critical dietary option for individuals with chronic diseases and are gaining favor among broader consumer groups. Consequently, the application of grains and cereals in baking premixes holds significant importance. This article reviewed the nutritional composition and efficacy of cereal and coarse grain premixed powders, examined their specific forms in food premixes, and emphasized the research progress regarding the food quality of cereal and coarse grain premixed powder products. Optimizing the particle size of coarse grain powders, adjusting formula compositions, and incorporating compound fermentation agents can effectively enhance the product quality and nutritional value of cereal and coarse grain baking premixed powders. Simultaneously, these methods improve the sensory experience of the final product, aligning it more closely with emerging trends in future food development. Grains and cereals, as potential daily dietary staples, possess the capability to comprehensively enhance food in terms of appearance, functionality, convenience and sensory enjoyment when formulated as ready-mixed powders. They are anticipated to become one of the key directions for the future development of the food industry. This article offers a theoretical foundation and technical guidance for the healthy and diversified advancement of the baking industry.

Objective To analyze the overall situation of food safety supervision and sampling inspection in an eastern coastal city from 2021 to 2024 and the main food safety issues. Methods The municipal level food safety supervision and sampling inspection data publicly disclosed by the eastern coastal city from 2021 to 2024 were aggregated. The overall inspection situation was analyzed using the dynamic trend of non-compliance rates, and the distribution characteristics of non-compliant samples across dimensions such as testing items, food categories, packaging factors and locally produced foods were stratified to identify the main factors affecting regional food safety. Results Over the past 4 years, a total of 34545 batches were inspected at the municipal level, with an overall non-compliance rate of 2.20%. The annual non-compliance rates fluctuated, showed a narrowing trend in amplitude. The highest non-compliance rates were observed in fruit products (5.83%), edible agricultural products (3.71%) and aquatic products (2.58%). In industrial processed foods, the non-compliance rates of bulk (2.01%) and pre-packaged (0.78%) showed a significant difference. The non-compliance rate of locally produced foods decreased fluctuatingly. Among them, 12 major categories (38.71%) showed no non-compliance for 4 consecutive years, while 13 categories (41.94%) exhibited a decline in non-compliance rates. Conclusion The overall status of food safety in this city showed a positive trend, with particularly quality improvement of locally produced foods. However, there were still risks in edible agricultural products and bulk foods. The regulators should pay more attention to them.

Objective To establish a method for determination of patulin in rice bran by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The patulin from rice bran samples were extracted with ethyl acetate, purified by a solid phase extraction cartridge, eluted with the mobile phase of 0.1% formic acid solution and acetonitrile, separated on the Luna®Omega Polar C₁₈ column (100 mm×2.1 mm, 3 μm), then analyzed in positive and negative ion mode of electrospray ionization source under multiple reaction monitoring mode, quantified by matrix matching curve external standard. Results In the range of 25.0-250.0 μg/L, the peak area of patulin was linearly correlated with the content, and the correlation coefficient (r²) was 0.9993. The limit of detection was 3.0 μg/kg, limit of quantitation was 10.0 μg/kg. Meanwhile, the average recoveries of patulin in rice bran were 86.7%-96.5% with the maximum relative standard deviations of 6.2% at the spiked concentration of 25.0, 50.0, 250.0 μg/kg. Conclusion This study establishes an efficient detection method for patulin in rice bran. This method has high precision and accuracy, and is simple to operate and has high analytical efficiency at the same time. The research results provide technical support for formulating the determination standard of patulin in rice bran and related quality control specifications.

Objective To explore the process of caffeine removal from green tea by supercritical carbon dioxide extraction. Methods Using Wuyuan green tea as raw material, supercritical carbon dioxide extraction was used to remove caffeine from green tea. Taking the caffeine removal rate as the investigation index, the effects of tea moisture content, extraction temperature, pressure, time, concentration and dosage of entrained agent on caffeine removal rate were studied by single factor experiment, and the caffeine removal process was optimized by orthogonal experiment. Results The optimal extraction process of caffeine removal in green tea was tea moisture content 40%, extraction pressure 30 MPa, extraction temperature 55 ℃, extraction time 240 min, concentration of entrainment agent (ethanol) 60%, and dosage of entrainment agent 1.2:1 (m:m). Under these conditions, the caffeine removal rate was 91.2%. Extraction pressure was the most important factor affecting caffeine removal rate, followed by extraction temperature, and extraction time had relatively little effect. Conclusion This study determines the best technology of caffeine removal in green tea by supercritical carbon dioxide extraction, which provides a certain reference for caffeine removal in green tea.

Objective To establish a method for the determination of 17α-estradiol and 17β-estradiol in aquatic products by QuEChERS combined with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The sample was extracted with water-acetonitrile (2:8, V:V), purified with N-propylethylenediamine, C₁₈ and neutral alumina, blown to dryness with nitrogen, and then reconstituted with water-acetonitrile (8:2, V:V). The sample solution was analyzed using water and acetonitrile (both containing 5 mmol/L ammonia) as mobile phase, separated by C₁₈ chromatographic column, scanned and detected in multiple reaction monitoring (MRM) mode, and quantified by external standard method. Results The results showed that 17α-estradiol and 17β-estradiol showed a good linear relationship in the concentration ranges of 5 to 200 μg/L, and the correlation coefficients were 0.9998 and 0.9985, respectively. The average recoveries at three spiked concentration levels varied from 66.5% to 101.1% with relative standard deviations of 0.62% to 10.17%. The limit of detection was 0.85-0.90 μg/kg, and the limit of quantitation was 2.1-2.4 μg/kg. Conclusion This method is simple, fast, sensitive and accurate, suitable for qualitative and quantitative analysis of 17α-estradiol and 17β-estradiol in aquatic products such as fish, shrimp, crabs and shellfish.

Objective To investigate the current situation of cadmium contamination in food and explore the dietary exposure risk of cadmium in the daily diet of residents in the southeast coastal areas. Methods Random sampling was conducted in major large-scale agricultural markets, commercial supermarkets, restaurants and other places throughout the city, with common commercially available foods as the research object. A total of 1285 food samples were collected from 7 categories, including grains and their products, vegetables, aquatic animals and their products, meat products, nuts and seeds, fresh edible fungi and seasonings. Inductively coupled plasma mass spectrometry method (ICP-MS) was applied for quantitative detection of cadmium content. The single factor pollution index (P_i) and target hazard factor (THQ) were used to evaluate the quality and consumption safety of each category of samples. Results After statistical analysis, there were significant differences in cadmium content among different categories of food. In the tested samples, the total detection rate of cadmium was 54.24%, and the detection result was undetected-2.86 mg/kg. Among them, crustaceans (sea crabs, shrimp) had the highest average detection value (1.2264 mg/kg). Conclusion The dietary exposure risk assessment results of tested sample indicate THQ<1. Therefore, the cadmium contamination in this region shows a low health risk to residents.